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KMID : 0380020100250050453
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 5 p.453 ~ p.458
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Development of Nanoenzymes for the Production of Glucose from Seaweed and Various Polysaccharide
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Kim Lie-Hua
Lee Jung-Heon
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Abstract
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The magnetically separable polyaniline nanofiber enzymes were developed for the recycle of enzyme and enhanced enzyme stability. The stability of enzyme was maintained over 90% for 8 days under room temperature and vigorous shaking conditions (200 rpm). The residual activity of immobilized enzyme was over 60% after 8 days incubation at 55℃. Glucose was produced from various polysaccharides, agarose, curdlan, cellulose, and sea weed, using magnetically separable immobilized enzyme. Glucose production rate with curdlan was 1.2 g/(l h) and showed high decomposition rate due to high mass transfer. After 10 times recycle, the residual activity of immobilized enzyme was over 75%. 1 g/L of glucose was produced with 5 mg of immobilized enzymes.
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KEYWORD
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nanoenzyme, seaweed, enzyme stability, glucose production
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